Here we align a set of sequences using the ClustalW option. You can create a multiple sequence alignment in MEGA using either the ClustalW or Muscle algorithms. Select the "hsp20.fas" file from the MEG/Examples directory. įrom the Alignment Explorer main menu, select Data | Open | Retrieve sequences from File. A dialog will appear asking ∺re you building a DNA or Protein sequence alignment? Click the button labeled DNA . Select Create New Alignment and click Ok. Launch the Alignment Explorer by selecting the Align | Edit/Build Alignment on the launch bar of the main MEGA window. The Alignment Explorer is the tool for building and editing multiple sequence alignments in MEGA. The location for Mac users is $HOME/MEGA/Examples, where $HOME is the users home directory ). All of the data files used in this tutorial can be found in the MEGA \ Examples \ folder (The default location for Windows users is C:\Program Files\MEGA\Examples \. In this tutorial, we will show how to create a multiple sequence alignment from protein sequence data that will be imported into the alignment editor using different methods. Creating Multiple Sequence Alignments Aligning Sequences
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May 2023
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